https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:40951 Wed 20 Jul 2022 15:22:13 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:35880 Tue 17 Dec 2019 17:03:41 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:42048 a]pyrene and benzo[a]pyrene NERs from the solvent-spiked soils, and mobilization of six PAHs and their NERs from the MGP soils. PAH oral bioaccessibility was determined. The incremental lifetime cancer risks (ILCRs), using Si-Org-PBET- and total-extractable PAH concentrations from the MGP soils, were calculated. Sorption kinetics modelling showed that 95% of mobilized PAHs sorbed to the silicone rods within 2–19 h, depending on PAH physico-chemical properties. Total-extractable and Si-Org-PBET extractable PAH concentrations exceeded health investigation levels (3 mg/kg based on benzo[a]pyrene toxic equivalent quotients) in soils. PAH oral bioaccessibility approached 100% for solvent-spiked soils, but only 24–36% for the MGP soils. Associated ILCRs exceeded target levels (10⁻⁵) for one MGP soil, particularly for 2–3 year olds, despite oral bioaccessibility considerations. In contrast, mobilized PAH NERs did not exceed health investigation and ILCR levels, as the NERs were highly sequestered, especially in the MGP soils. PAH nonextractable residues in long-term contaminated soils are unlikely to be mobilized in concentrations that pose cancer risks to humans following soil ingestion, and do not need to be considered in risk assessments.]]> Tue 14 Nov 2023 14:48:37 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:43101 40%) amounts of nonextractable residues. The cumulative amounts of B[a]P extracted in each soil by single- and sequential-solvent extractions were similar (p > 0.05) at each aging period, which indicated access to similar B[a]P fractions in soil by both solvent extractions. The similarities in the amounts of B[a]P nonextractable residues recovered by MeKOH from pre-extracted soils, through either of the extraction methods, confirms that the total extractable B[a]P fractions from both methods are similar.]]> Tue 13 Sep 2022 12:40:51 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:35161 Zea mays L.) metabolome on exposure to high molecular weight PAHs such as benzo[a]pyrene (BaP) and pyrene (PYR) in a hydroponic medium, individually and as a mixture (BaP + PYR) using GC-MS. The differences in the metabolites were analyzed using XCMS (an acronym for various forms (X) of chromatography-mass spectrometry), an online-based data analysis tool. A significant variation in metabolites was observed between treatment groups and the unspiked control group. The univariate, multivariate and pathway impact analysis showed there were more significant alterations in metabolic profiles between individual PAHs and the mixture of BaP and PYR. The marked changes in the metabolites of galactose metabolism and aminoacyl tRNA biosynthesis in PAHs treated maize leaves exhibit the adaptive defensive mechanisms for individual and PAHs mixture. Therefore, the metabolomics approach is essential for an understanding of the complex biochemical responses of plants to PAHs contaminants. This knowledge will shed new light in the field of phytoremediation, bio-monitoring, and environmental risk assessment.]]> Tue 13 Apr 2021 09:16:53 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:48156 Thu 09 Mar 2023 09:44:46 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30891 14C-tracing study showed that the mild acid/alkali-treated clay products increased the PAH biodegradation (5–8%) in the order of 0.5 M HCl ≥ unmodified > 3 M NaOH ≥ 0.5 M NaOH for smectite, and 0.5 M HCl > 0.5 M NaOH ≥ unmodified ≥ 3 M NaOH for palygorskite. The biodegradation was correlated (r = 0.81) with the bioavailable fraction of PAHs and microbial growth as affected particularly by the 0.5 M HCl and 0.5 M NaOH-treated clay minerals. These results could be pivotal in developing a clay-modulated bioremediation technology for cleaning up PAH-contaminated soils and sediments in the field.]]> Sat 24 Mar 2018 07:30:38 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:23141 Sat 24 Mar 2018 07:10:40 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:49847 Mon 05 Jun 2023 15:51:30 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:34241 Fri 22 Feb 2019 14:42:10 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:49151 Fri 05 May 2023 12:20:55 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:52970 Fri 03 Nov 2023 15:30:26 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:34310 Eisenia fetida is a sentinel organism in soil which can be used to diagnose the health of the soil. In order to identify potential molecular markers from Eisenia fetida to diagnose the presence of benzo(a) pyrene in soil, we exposed the organism to sub-lethal (10 mgKg^-1) concentrations for a period of eight months and carried out transcriptome analysis. From the transcriptome, we have identified differentially expressed genes. Results showed that benzo(a)pyrene has altered the expression of calcium binding and calcium homeostasis, apoptotic process, cytoskeletal proteins, protein transport, nucleotide binding, lipid metabolism, peripheral neuronal development, cell division, wound healing and processing genes at transcription level. Several of the genes we reported here were not reported earlier. The highly up regulated and down regulated genes could be used as a molecular marker to diagnose the presence of benzo(a)pyrene in the soil.]]> Fri 01 Apr 2022 09:28:26 AEDT ]]>